| No. Registro | 002921572 |
| Tipo de material | ARTIGO DE PERIODICO - INTERNACIONAL |
| Cód. publicação |  10.3389/fphar.2018.01191 DOI |
| Entrada Principal | Caldas, Lhaís Araújo (*) NAC Brasil |
| Título | Calein C, a Sesquiterpene Lactone Isolated From Calea Pinnatifida (Asteraceae), Inhibits Mitotic Progression and Induces Apoptosis in MCF-7 Cells. |
| Imprenta | Lausanne, 2018. |
| Descrição | p. 1-10. |
| Idioma | Inglês |
| Nota | Disponível em: <http://dx.doi.org//10.3389/fphar.2018.01191>. Acesso em: 22 de jan. 2019 |
| Ag de Financiamento | Financiado pela FAPESP 2016/24985-4 |
| | Financiado pela FAPEMIG APQ-02810-16 |
| | Financiado pela CAPES |
| | Financiado pelo CNPq |
| Assunto | NEOPLASIAS |
| | MAMA |
| | COMPOSITAE |
| | QUIMIOTERÁPICOS |
| | FITOQUÍMICA |
| | PRODUTOS NATURAIS |
| Assunto | Asteraceae |
| | Calea pinnatifida |
| | Calein C |
| | Antimitotic activity |
| | Breast cancer |
| | AURKB |
| | PLK-1 |
| Autor Secundário | Horvath, Renato O (*) NAC Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Brazil Brasil |
| | Ferreira-Silva, Guilherme Álvaro (*) NAC Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Brazil Brasil |
| | Ferreira, Marcelo J. Pena |
| | Ionta, Marisa (*) NAC Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Brazil Brasil |
| | Sartorelli, Patricia (*) NAC Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil Brasil |
| Fonte | In: Frontiers in Pharmacology, Lausanne, v. 9, art. 1191, p. 1-10, Oct. 2018, ISSN: 1663-9812 |
| Localiz.Eletrônica |
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| Resumo/Outros | Breast cancer is the most common cancer in women worldwide. Estrogen receptor-positive (ER+) breast cancer represents approximately 75% of diagnosed cases, while 15–20% of them are triple-negative (TN). Although there have been improvements in the therapeutic approach, the mortality rate remains elevated. Thus, it is necessary to identify new chemotherapeutic agents. The present study aimed to evaluate the effects of calein C, a sesquiterpene lactone isolated from Calea pinnatifida, on breast cancer cell lines MCF-7 (ER+), Hs578T (TN) and MDA-MB-231 (TN). Calein C significantly reduced the viability of all cell lines; however, MCF-7 cells were more responsive than MDA-MB-231 or Hs578T cells. Thus, the MCF-7 cell line was selected for further investigation. We demonstrated that calein C inhibited cell cycle progression in MCF-7 cells at M-phase. Increased frequency of mitosis was observed in calein C-treated samples compared to the control group, especially of the cell population in initial stages of the mitosis. These events were associated with the ability of calein C to modulate expression levels of critical regulators of mitosis progression. We observed a significant reduction in the relative mRNA abundance of PLK1 and AURKB along with a concomitant increase in CDKN1A (p21) in treated samples. In addition, calein C induced apoptosis in MCF-7 cells due to, at least in part, its ability to reduce the BCL2/BAX ratio. Therefore, our data provide evidence that calein C is an important antimitotic agent and should be considered for further in vivo investigations |
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| Unidade USP | IB -- INST DE BIOCIÊNCIAS |
