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Registro Completo

Escolher formato: Padrão Ficha Formato Reduzido Nomes MARC Campos MARC
No. Registro   002837325
Tipo de material   TRABALHO DE EVENTO-RESUMO PERIODICO - INTERNACIONAL
Entrada Principal   LinkMulyar, Y. (*) INT Canadá
Título   LinkZika virus peptides identification in saliva after 9-months infection.
Imprenta   Alexandria : International Association for Dental Research, 2017.
Descrição   abst. 1639.
Idioma   Inglês
Nota Local   MPC Digital
Assunto   LinkSALIVA -- VIROLOGIA
  LinkZIKA VÍRUS
  LinkPROTEÍNAS
Assunto   LinkProteome
  LinkProteomics
Autor Secundário   LinkJorge, P. K. (*) NAC Pediatric Dentistry, Bauru Dental School, University of São Paulo, Bauru, SP Brasil
  LinkZuanazzi, D. (*) INT University of Western Ontario, London, Ontario Canadá
  LinkGibson, R. (*) INT University of Western Ontario, London, Ontario Canadá
  LinkXiao, Y. (*) INT University of Western Ontario, London, Ontario Canadá
  LinkSantos, M. Bringel dos (*) NAC Pediatric Dentistry, Bauru Dental School, University of São Paulo, Bauru, SP Brasil
  LinkArts, E. J. (*) INT University of Western Ontario, London, Ontario Canadá
  LinkMachado, Maria Aparecida de Andrade Moreira
  LinkSiqueira, W. L. (*) INT University of Western Ontario, London, Ontario Canadá
Autor Secundário   LinkGeneral Session & Exhibition of the IADR - International Association for Dental Research (95. 2017 São Francisco, CA)
  LinkAnnual Meeting of the AADR - American Association for Dental Research (46. 2017 São Francisco, CA)
  LinkAnnual Meeting of the CADR - Canadian Association for Dental Research (41. 2017 São Francisco, CA)
Fonte   LinkIn: Journal of Dental Research, v. 96, Sp. Issue A, abst. 1639, 2017, ISSN: 0022-0345
Localiz.Eletrônica    "Clicar" sobre o botão para acesso ao Currículo Lattes de Maria Aparecida de Andrade Moreira Machado 
Resumo/Outros   Objectives: To demonstrate the applicability of a mass spectrometry-based proteomic technique for identification of Zika virus (ZIKV) peptides in saliva of patients previously diagnosed with ZIKV. Methods: A 25-year old woman clinically diagnosed with ZIKV infection in the first trimester gave birth to dizygotic twins, baby-A (female) had microcephaly while the baby-B (male) did not have microcephaly. Saliva was collected from mother and babies 3 months after birth (a total of 9-months post ZIKV infection). Peptidomic analysis was completed by mass spectrometry. MS/MS data was searched against a specific ZIKV protein database. Identified ZIKV peptides were aligned/mapped to the ZIKV polyprotein regions. Lastly, phylogenetic analyses were generated from the peptidomic data. Results: We could identify ZIKV peptides in saliva 9 months after infection. A total of 420 (mother), 670 (baby-A), and 183 (baby-B) ZIKV peptides were identified in saliva, which covered 67% (mother), 84% (baby A), and 45% (baby-B) of the ZIKV polyprotein. A significant shift was noted in the number of amino acid residues per peptide on baby-A (female affected by microcephaly) when compared to mother and baby-B (male not affected by microcephaly). Additionally, both mother and baby A demonstrated similar structural/non-structural ratio, whereas baby B showed a slightly reduced ratio of structural peptides. Conclusions: Timely diagnosis of ZIKV infection can allow us to act decisively and take preventative measures in terms of containing the spread of Zika virus infection. Thus, we introduce an innovative approach to assessing the presence of ZIKV peptides in saliva. Moreover, our data strongly confirms a vertical transmission (mother to babies). We can definitively conclude that our novel approach of looking at natural occurring peptides from ZIKV increases the window of detection when compared to RNA-based diagnostics
 
Unidade USP   FOB -- FAC DE ODONTOLOGIA DE BAURU

Escolher formato: Padrão Ficha Formato Reduzido Nomes MARC Campos MARC


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